Open Access Journals: Recent submissions
Now showing items 661-680 of 831
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Research of total levels on DNA methylation in plant based on HPLC analysis
(Scientific Research Publishing, 2013-04)HPLC analysis is important for determination of total level on DNA methylation in plants. It can be used to help characterise epigenetic changes during growth, development and stress. HPLC methods have been optimised for ... -
1,25-Dihydroxyvitamin D3 effects on the regulation of the insulin receptor gene in the hind limb muscle and heart of streptozotocin-induced diabetic rats
(Scientific Research Publishing, 2013-04)In the present study, we examine the effects of the treatment with 1,25-dihydroxyvitamin D3 [150 IU/Kg (3.75 μg/Kg) once a day, for 15 days] to non-diabetic and streptozotocin-induced diabetic rats. The results indicate ... -
Breeding of NHase hyper-producing Rhodococcus ruber strain LUV30-06 and verification of mutants by RAPD
(Scientific Research Publishing, 2013-04)A nitrile hydratase (NHase) hyper-producing Rhodococcus ruber strain LUV30-06 was bred by mutagenization on the starting strain CGMCC3090 with ultraviolet irradiation and lithium chloride. The NHase activity of the strain ... -
Plasmid instability when the hsp60 gene promoter is used to express the protective non-toxic fragment B of the diphtheria toxin in recombinant BCG
(Scientific Research Publishing, 2013-04)The genetic modification of the live attenuated Mycobacterium bovis BCG to deliver a protective Corynebacterium diphtheriae antigen in vivo could be a safer and less costly alternative to the new and more expensive DTP ... -
Effect of SP600125 on proliferation of embryonic stem cell
(Scientific Research Publishing, 2013-04)SP600125 is an inhibitor of c-Jun NH2-terminal kinase (JNK), which plays a fundamental role in regulating animal development. Using SP600125 to deal with the mouse embryonic stem cells, it is revealed that the number of ... -
The molecular regulatory effect of intracerebroventricular thymulin on endotoxin-mediated NF-kB nuclear translocation and activation in vivo
(Scientific Research Publishing, 2013-01)The nuclear factor-kB (NF-kB) is one member of a ubiquitously expressed family of Rel-related transcription factors that serve as critical regulators of proinflammatory genes. The immunomodulatory potential of thymulin and ... -
Characterization of the role of Smu1 in nuclear localization of splicing factors in the mammalian temperature-sensitive mutant
(Scientific Research Publishing, 2013-01)A temperature-sensitive (ts) mutant of the CHO-K1 cell line, tsTM18, grows at 340C but not at 390C. Smu1 is the gene responsible for ts defects of tsTM18 cells. Previously, we found that the Smu1 ts defect altered the ... -
Identification and characterization drought tolerance of gene LEA-D11 soybean (glycine max L. Merr) based on PCR-sequencing
(Scientific Research Publishing, 2013-01)Drought is one of the most damaging abiotic stress. Different plants response differently to drought stress. Abiotic stresses such as drought induced diverse physicological and molecular responses in plants. These responses ... -
Using Bayesian and Eigen approaches to study spatial genetic structure of Moroccan and Syrian durum wheat landraces
(Scientific Research Publishing, 2013-01)The Mediterranean durum wheat landraces are genetically diverse and important sources for improving resistance to abiotic and biotic stresses and developing adapted and productive durum wheat varieties in the Mediterranean ... -
Frequently cutting restriction enzymes: Clearing the fog to see the ends
(Scientific Research Publishing, 2013-01)Telomeres, which are found at the ends of eukaryotic chromosomes, are composed of tandem arrays of repetitive sequences and safeguard genomic stability. Previous studies have revealed that telomeric repeats are also present ... -
Bax promoter G(－248)A polymorphism in a Turkish clinical breast cancer patients: A case-control study
(Scientific Research Publishing, 2013-01)Bax is an important protein involved in apoptotic process. Mutations of the Bax gene are known to af- fect protein expression and function. A promotor polymorphism G(－248)A in the 5’UTR of Bax gene may alter regulation of ... -
Effect of 4% glycerol and low aeration on result of expression in Escherichia coli of Cin3 and three Venturia inaequalis EST’s recombinant proteins
(Scientific Research Publishing, 2013-01)The phytopathogenic fungus Venturia inaequalis causes scab of apple. Once this fungus penetrates the plant surface, it forms a specialized body called a stroma between the inner cuticle surface and the epidermal cell wall. ... -
GAPDH expression as a measurement of transfection efficiency for p16INK4a gene silencing (siRNA) in senescent human diploid fibroblasts
(Scientific Research Publishing, 2012-10)Human diploid fibroblasts (HDFs) undergo a limited number of cell divisions in culture. After certain population doublings, they reach a state of irreversible growth arrest known as replicative senescence. Senescent HDFs ... -
Genetic variation may play a crucial role in non-coding RNA biogenesis
(Scientific Research Publishing, 2012-10)Transcription, post-transcriptional modification, translation, post-translational modification, DNA replication, and signaling interaction of intra- and extra- cellular components are the relevant mechanisms in gene ... -
A simple and efficient seed-based approach to induce callus production from B73 maize genotype
(Scientific Research Publishing, 2012-10)The wild type maize genotype, B73, is not amenable for callus production and an efficient protocol for B73 maize callus induction has never been reported up-to-date. Scientific efforts in producing B73 maize callus using ... -
An engineered Phlebia radiata manganese peroxidase: expression, refolding, purification and preliminary characterization
(Scientific Research Publishing, 2012-10)Manganese peroxidases (MnPs) are interesting enzymes in protein engineering, aimed at maximizing industrial bioprocesses such as lignin degradation and biofuel production. cDNA of the secreted short-type of MnP from Phlebia ... -
Chimeric oncogenic interferon regulatory factor-2 (IRF-2): Degradation products are biologically active
(Scientific Research Publishing, 2012-10)Interferon Regulatory Factor-2 (IRF-2) belongs to IRF family, was identified as a mammalian transcrip-tion factor involved in Interferon beta (IFNβ) gene regulation. Besides that IRF-2 is involved in ... -
A silkworm hemolymph protein is a prophenoloxidase activation blocker
(Scientific Research Publishing, 2012-10)Melanization in insect hemolymph is triggered by the recognition of pathogen-associated molecular patterns via pattern recognition receptors. The signal transduction leads to the activation of the prophenoloxidase and hence ... -
Mutated elements of a complex promoter (Amh) can help to demonstrate the role of certain elements in controlling differential gene expression
(Springer Science+Business Media, 2012-10)Amh is a single copy gene which is expressed in different ways during mammalian development. Several potential promoter elements have been identified using physiological experimentation and on the basis of interspecific ... -
Biocatalytic production of chitosan polymers from shrimp shells, using a recombinant enzyme produced by pichia pastoris
(Scientific Research Publishing, 2012-10)Chitosan has a unique chemical structure with high charge density, reactive hydroxyl and amino groups, and extensive hydrogen bonding. Chitin deacetylase (EC 3.5.1.41) catalyzes the hydrolysis of the N-acetamido groups of ...
