Abstract:
Degradation of cellulose by fungi is a scientifically established phenomenon. This research a
successful isolated five fungal isolates named OV1, OV2, OV3 and OV4 were obtained from
dead decaying wood at the University of Embu. Samples were collected from the forest and
taken to the laboratory where different tests were performed. The isolates were exposed at p.H
of 3.2, 8.5, and 7.0 whereby different growth characteristics were exhibited. Temperatures of
42
0
0
C, 10
0
C and 28
C were used in checking the degrading ability of these these isolates
showed different degradation rates. Skimmed milk, citrate, starch, xylose and glycerol
substrates were as well used as substrates to be utilized by this isolates. In this substrate
utilization growth occurred in some and failed in other substrates. Morphological identification
including mycelium color, colony characteristics and microscopic features were used when
colonies were grown on PDA. Biochemical and physiological tests were as well done to
differentiate between the isolates ability to degrade cellulose and the optimum conditions
required for growth. of isolates.OV1 is postulated to belong to Beauveria spp, OV2 to
Aspergillus spp, OV3 to Trichorderma spp and OV4 to Verticillium spp.. The research
facilitated the acquisition of knowledge on the decomposition process that is aided by cellulose
degrading fungi in the University of Embu forest. Therefore the knowledge obtained in
understanding the degradation mechanisms and process of complex molecules (cellulose) by
fungi can be applied in the management of organic wastes.
